Surface Enhanced CdSe/ZnS QD/SiNP Electrochemical Immunosensor for the Detection of Mycobacterium Tuberculosis by Combination of CFP10-ESAT6 for Better Diagnostic Specificity.

摘要: In this study, an electrochemical immunosensor was introduced for the detection of tuberculosis (TB) via utilization a modified electrode containing quantum dot (CdSe/ZnS QD) and functionalized silica nanoparticles (SiNPs) on screen-printed carbon (SPCE) CdSe/ZnS QD/SiNPs/SPCE, by employing indirect enzyme-linked immunosorbent assay (ELISA). Here, fabricated linked to biocatalytic action enzyme catalase through antigen-antibody binding antigen (CFP10-ESAT6) means producing differential pulse voltammetry (DPV) current. The characterization cyclic (CV) showed good behavior enhanced high electron transfer between analyte. Moreover, active surface area 4.14-fold higher than bare SPCE. developed method selectivity towards CFP10-ESAT6 compared with other TB proteins. also linear response different concentrations R2 = 0.9937, yielding limit (LOD) as low 1.5 × 10-10 g/mL range 40 100 ng/mL concentration. proposed reproducibility target analyte relative standard deviation 1.45%.