Cathepsins B and D are dispensable for major histocompatibility complex class II-mediated antigen presentation

摘要: Antigen presentation by major histocompatibility complex (MHC) class II molecules requires the participation of different proteases in endocytic route to degrade endocytosed antigens as well MHC II-associated invariant chain (Ii). Thus far, only cysteine protease cathepsin (Cat) S appears essential for complete destruction Ii. The enzymes involved degradation themselves remain be identified. Degradation vitro and experiments using inhibitors have suggested that Cat B D, two aspartyl proteases, respectively, are antigen degradation. We analyzed antigen-presenting properties cells derived from mice deficient either or D. Although absence these provoked a modest shift efficiency some antigenic determinants, overall capacity B-/- D-/- was unaffected. Ii proceeded normally splenocytes, it did cells. conclude neither nor D II-mediated presentation.