A Flowthrough Infusion Calorimeter for Measuring Muscle Energetics: Design and Performance

摘要: The heat produced during muscle contraction arises from distinct underlying cellular processes. While current calorimeters can measure total production, none allow accurate titration and infusion of chemical drugs to probe the thermodynamic mechanisms that underlie various heart diseases. We have constructed a calorimeter incorporates an system for exposing samples time-varying concentrations pharmacological agents. design instrument is informed by development use analytical lumped parameter finite-element models. This new uses thermopile-based sensors achieve noise-equivalent power 2.6 $\text {nW}\cdot \text {Hz}^{ {-1/2}}$ signal-to-noise ratio up approximately 1700. report on modeling optimization techniques were used inform our design. characterize instrument’s performance demonstrate its utility interventions mimic two cardiac disease conditions, acute force impairment dynamic contracture.