Bcl-2 protects against apoptosis-related microtubule alterations in neuronal cells.
作者： Rony Nuydens , Gwenda Dispersyn , Gerd Van Den Kieboom , Mirjam De Jong , Rich Connors
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摘要: Bcl-2 is a gene with clear anti-apoptotic properties in neurodegenerative conditions. One of the earliest hallmarks degeneration neuronal cell cultures loss neurite morphology. Therefore effect on morphology and microtubule stability was studied nerve growth factor differentiated PC12 cells. Microtubule dynamics were modulated using stabilizer taxol destabilizer, okadaic acid, protein phosphatase inhibitor. It shown that protects against both taxol- acid induced retraction. overexpression also significantly reduced increased ratio acetylated tubulin over total by treatment. Interestingly, attenuates decrease same after exposure to suggesting able normalize level tubulin. In addition, death nuclear fragmentation, overexpressing This protection either downstream or independent tau phosphorylation as quantitative immunocytochemistry AT8 showed did not modify phosphorylation. The data suggest protective cytoskeleton probably linked changes post-translational modification
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