Interaction of lactoferrin, monocytes, and T lymphocyte subsets in the regulation of steady-state granulopoiesis in vitro.

摘要: Abstract Colony-stimulating activities (CSA) are potent granulopoietic stimulators in vitro. Using clonogenic assay techniques, we analyzed the degree to which mononuclear phagocytes and T lymphocytes cooperate positive (production/release of CSA) feedback (inhibition CSA production/release) regulation granulopoiesis. We measured effect lactoferrin (a putative regulator production) on provision three separate systems wherein granulocyte colony growth marrow cells from 22 normal volunteers was stimulated by (a) endogenous CSA-producing suspension, (b) autologous peripheral blood leukocytes feeder layers, (c) medium conditioned leukocytes. The cell populations each were varied using separation techniques exposure isolated methylprednisolone or monoclonal antibodies surface antigens complement. noted that net production increased more than twofold when a small number unstimulated added monocyte cultures. Lactoferrin's inhibitory also lymphocyte dependent. interact with monocytes inhibit similar those augment because their neither genetically restricted not glucocorticoid sensitive, both express HLA-DR (Ia-like) T3 but T4 T8 antigens. These findings consistent results our studies mechanism lactoferrin's indicate produce soluble factors stimulate CSA, does suppress production, completely release CSA. conclude subset exhibit important complex interactions