Fabrication of shish–kebab structured poly(ε-caprolactone) electrospun nanofibers that mimic collagen fibrils: Effect of solvents and matrigel functionalization

摘要: Abstract Surface properties of tissue engineering scaffolds such as topography, hydrophilicity, and functional groups play a vital role in cell adhesion, migration, proliferation, apoptosis. In this study, poly(e-caprolactone) (PCL) shish–kebab (PCL-SK), which feature three-dimensional structure comprised electrospun PCL nanofibers covered by periodic, self-induced crystal lamellae on the surface, was created to mimic nanotopography native collagen fibrils extracellular matrix (ECM). Two different kinds solvents used creating kebabs shish were investigated real-time observation. It found that solvent solubility directly affects formation kebabs: lower solvent, easier surface nanofibers. addition, matrigel covalently immobilized alkaline hydrolyzed PCL-SK enhance their hydrophilicity. This combined approach not only mimics fibrils, but also simulates features for growth. To investigate viability scaffolds, HEF1 fibroblast assays conducted results revealed facilitated adhesion proliferation. The functionalization further enhanced cellular response, suggested elevated biocompatibility greater potential applications.