Laser Ablation In Arabidopsis Roots: A Tool To Study Cell-To-Cell Communication

摘要: Developmental processes have been widely studied by manipulations of cells and tissues in intact organisms. These techniques are only the last decades being complemented powerful approach genetic manipulations. In animals, several experimental tools deployed that directed towards unravelling cause differential cell fate. First, transplantation experiments or to heterotropic sites can reveal many inductive a well known example was 1924 Spemann Mangold experiment using two differently pigmented species newt. A transplanted dorsal blastophore lip into host embryo induced second jointed its host, showing axis formation reveals multiple (reviewed Gilbert, 1988). Second, disconnection blastomeres at early embryonic stages shows whether fate determinants spatially separated (Laufer et al., 1980; Priest Thomson, 1987). Third, injection vital dyes single labels descendants (clonal analysis), what stage committed still contribute distinct types (Keller 1975, 1976).