Phylogenetic analysis of homologous fatty acid synthase and polyketide synthase involved in aflatoxin biosynthesis.

摘要: The first two steps of aflatoxin biosynthesis are catalyzed by the HexA/B and Pks protein. phylogenetic analysis clearly distinguished fungal from FAS subunits other homologous proteins. trees HexA HexB set proteins share same clustering. Proteins involved in synthesis fatty acids or sterigmatocystin cluster separately. tree also differentiates aflatoxin-related polypeptide sequences those kinds secondary metabolism. function some A. flavus homologues may be deduced analysis. conserved sequence motifs protein domains shared - namely, β-polyketide synthase (KS), acetyl transferase (AT) acyl carrier (ACP) have been identified, primary metabolism