Oxidative DNA damage by t-butyl hydroperoxide causes DNA single strand breaks which is not linked to cell lysis. A mechanistic study in freshly isolated rat hepatocytes.
作者： I. Latour , J.B. Demoulin , P. Buc-Calderon
DOI: 10.1016/0014-5793(95)01065-M
关键词:
摘要: In rat hepatocytes, DNA damage by t-butyl hydroperoxide (tBOOH) was measured using the fluorimetric analysis of alkaline unwinding. The electrophoretic profile genomic suggests single rather than double strand breaks formation. Oxidative modifications, as increased 8-hydroxy-deoxyguanosine content, were not detected. Lysis hepatocytes and induced tBOOH did correlate, indicating that both processes are interconnected. Since o-phenanthroline prevents against tBOOH-mediated effects on membrane integrity, we discussed about a putative role iron.
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sci-hub.se 参考文章(32)
Daniel C. Harris, Quantitative Chemical Analysis ,(1982)
J L Farber, A P Thomas, I Sakaida, Increases in cytosolic calcium ion concentration can be dissociated from the killing of cultured hepatocytes by tert-butyl hydroperoxide. Journal of Biological Chemistry. ,vol. 266, pp. 717- 722 ,(1991) , 10.1016/S0021-9258(17)35230-4
David J. McConkey, Pia Hartzell, Pierluigi Nicotera, Sten Orrenius, Calcium-activated DNA fragmentation kills immature thymocytes. The FASEB Journal. ,vol. 3, pp. 1843- 1849 ,(1989) , 10.1096/FASEBJ.3.7.2497041
R C Duke, J J Cohen, Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death. Journal of Immunology. ,vol. 132, pp. 38- 42 ,(1984)
C G Fraga, A L Tappel, Damage to DNA concurrent with lipid peroxidation in rat liver slices Biochemical Journal. ,vol. 252, pp. 893- 896 ,(1988) , 10.1042/BJ2520893
R. S. McWilliams, W. G. Cross, J. G. Kaplan, H. C. Birnboim, Rapid Rejoining of DNA Strand Breaks in Resting Human Lymphocytes after Irradiation by Low Doses of 60 Co g Rays or 14.6-MeV Neutrons Radiation Research. ,vol. 94, pp. 499- 507 ,(1983) , 10.2307/3575907
J.-F. Mouret, M. Polverelli, F. Sarrazini, J. Cadet, Ionic and radical oxidations of DNA by hydrogen peroxide Chemico-Biological Interactions. ,vol. 77, pp. 187- 201 ,(1991) , 10.1016/0009-2797(91)90073-G
J. Imlay, S Linn, DNA damage and oxygen radical toxicity Science. ,vol. 240, pp. 1302- 1309 ,(1988) , 10.1126/SCIENCE.3287616
Christopher H. Kennedy, William A. Pryor, Gary W. Winston, Daniel F. Church, Hydroperoxide-induced radical production in liver mitochondria. Biochemical and Biophysical Research Communications. ,vol. 141, pp. 1123- 1129 ,(1986) , 10.1016/S0006-291X(86)80160-7
M.A. Barry, A. Eastman, Identification of deoxyribonuclease II as an endonuclease involved in apoptosis Archives of Biochemistry and Biophysics. ,vol. 300, pp. 440- 450 ,(1993) , 10.1006/ABBI.1993.1060