Competitive immunoassay method, device and test kit

摘要: An immunoassay device including one or more reaction chambers, each adapted to receive and retain a volume of test fluid in communication with nonoverlapping first, second, third reagent-bearing surfaces. To the first surface is reversibly bound an analyte conjugate: component conjugated components, termed ligand/marker, that serve ligand marker functions as described herein. Analyte binding partner immobilized on second surface, ligand/marker surface. The chamber preferably configured direct sequentially past reagent In use, conjugate solubilized from completes any for sites Excess becomes sequestered where activity read indicate presence concentration fluid. A kit includes combination comparative results.