Expression and functional analysis of voltage-activated Na+ channels in human prostate cancer cell lines and their contribution to invasion in vitro.

摘要: Ion channels are important for many cellular functions and disease states including cystic fibrosis multidrug resistance. Previous work in the Dunning rat model of prostate cancer has suggested a relationship between voltage-activated Na+ (VASCs) invasive phenotype vitro. The objectives this study were to 1) evaluate expression VASCs LNCaP PC-3 human cell lines by Western blotting, flow cytometry, whole-cell patch clamping, 2) determine their role invasion vitro using modified Boyden chambers with without specific blocker (tetrodotoxin). A 260-kd protein representing was found only line, these shown be membrane expressed on cytometry. Patch clamping studies indicated that functional present 10% cells blocking tetrodotoxin (600 nmol/L) reduced invasiveness 31% (P = 0.02) affecting cells. These results indicate reduction is direct result VASC blockade not nonspecific action drug. This first report prostatic line. but as determined both studies. Tetrodotoxin cells, data suggest ion may play an tumor invasion.