BIOMARKERS FOR EARLY DETECTION OF GASTRIC CANCER BASED ON AUTOANTIBODIES SIGNATURES
作者： Diana Alexandra Vieira Campos
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摘要: 5 Resumo 7 Introduction 9 1. Mucins 10 1.1 Mucin structure 1.2 and cancer 11 2. OGlycosylation 12 2.1 Polypeptide α-GalNAc transferases 13 2.2 Sialyl-Tn antigen 14 2.3 Core 1 15 2.4 2 core 4 16 2.5 3 2.6 Elongation Synthesis of terminal structures 17 3. Alterations in mucin glycosylation as biomarker for detection 18 4. Rationale this work project 19 Objectives 21 Methods 23 recombinant glycoprotein fragments E.coli 25 Matrix-assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-TOF) ...... 26 O-glycopeptides O-glycopeptide array print anlysis 27 5. Monoclonal antibodies 28 6. Human sera
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