The effects of demineralisation and sampling point variability on the measurement of glutamine deamidation in type I collagen extracted from bone

作者: J.P. Simpson , K.E.H. Penkman , B. Demarchi , H. Koon , M.J. Collins

DOI: 10.1016/J.JAS.2016.02.002

关键词:

摘要: The level of glutamine (Gln) deamidation in bone collagen provides information on the diagenetic history but, order to accurately assess extent Gln deamidation, it is important minimise conditions that may induce during sample preparation. Here we report results a preliminary investigation variability levels an archaeological due to: a) sampling location within bone; b) localised diagenesis; and c) preparation methods. We then investigate effects pre-treatment three samples: one modern, Medieval Pleistocene. treatment with acidic solutions was found both break down fibril structure. This particularly evident Pleistocene material (∼80,000 years BP) considered this study. show ethylenediaminetetraacetic acid (EDTA), when used as alternative hydrochloric (HCl) demineralisation, induces minimal maintains Areas exhibiting degradation are shown be correlated increase deamidation. indicates could provide marker for diagenesis but important, that, whenever possible, subsamples should taken from areas visually representative whole. Although validation our observations will require analysis larger set, measurements valuable screening tool evaluate suitability further destructive analyses such isotopic or DNA analysis, well assessing overall preservation at site. measure useful help conservators identify bones special long-term storage conditions.

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