Mechanical production of pellets for the application of entomopathogenic nematodes: effect of pre-acclimation of Steinernema glaseri on its survival time and infectivity against Phyllophaga vetula

作者: Carlos Inocencio Cortés-Martínez , Edwin E. Lewis , Jaime Ruiz-Vega , Gabino Alberto Martínez-Gutiérrez

DOI: 10.1080/09583157.2017.1366423

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摘要: ABSTRACTThe low survival time and diminished infectivity by entomopathogenic nematodes (EPNs) from granular formulations limit their efficiency against agricultural insect pests. This study determined the benefit of pre-acclimating infective juveniles (IJs) Steinernema glaseri (NJ-43 strain) on extending mean (STm) in diatomaceous earth (DE) pellets increasing Phyllophaga vetula. The IJs were reared Galleria mellonella larvae placed Petri dishes containing plaster Paris (PP) or modified White traps (WTs). Pelletisation was performed a machine operating principle laminar flow using DE Celite® 209. Pellets stored at room temperature (23 ± 3°C) high relative humidity (96–100%). harvested WTs between 3rd 5th days after onset emergence more P. vetula pre-acclimation S. PP increased significantly its STm pellets; 23.1 to 34.5 days, compared with non-pre-...

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