Metabolomic profiling of oxalate-degrading probiotic Lactobacillus acidophilus and Lactobacillus gasseri.

摘要: Oxalate, a ubiquitous compound in many plant-based foods, is absorbed through the intestine and precipitates with calcium kidneys to form stones. Over 80% of diagnosed kidney stones are found be oxalate. People who these often experience high rate recurrence treatment options remain limited despite decades dedicated research. Recently, intestinal microbiome has become new focus for novel therapies. Studies have shown that select species Lactobacillus, most commonly included genus modern probiotic supplements, can degrade oxalate vitro even decrease urinary animal models Primary Hyperoxaluria. Although purported health benefits Lactobacillus probiotics vary significantly between species, there supporting evidence their potential use as diseases. Defining unique metabolic properties essential define how bacteria interact host influence overall health. We addressed this need by characterizing comparing metabolome lipidome oxalate-degrading acidophilus gasseri using ultra-high-performance liquid chromatography-high resolution mass spectrometry. report species-specific differences profiles discuss relevance function resulting from differential expression. Also described our validation ability gasseri, presence other preferred carbon sources, measuring 14C-oxalate consumption via scintillation counting.