Fluorescent Probe Encapsulated in Avidin Protein to Eliminate Nonspecific Fluorescence and Increase Detection Sensitivity in Blood Serum

摘要: Quantitative detection of trace amounts a biomarker in protein rich human blood plasma using fluorescent probes is great challenge as the real signal usually obscured by nonspecific fluorescence. This problem occurs because most dyes bind very tightly with proteins to produce large fluorescence increase, resulting overestimation concentrations and false positive diagnosis. In this paper, we report that biotinylated encapsulated avidin can generate specific serum blocking out dye–protein interactions. We applied our novel probe design detect two different types biomolecules, hydrogen sulfide nitroreductase. Our Avidin conjugated achieved quantitative analyte serum; whereas were overestimated up 320-fold when bare employed. As compared conventional approaches where are in...