Phototoxicity Mechanism of a Kryptocyanine Dye in Human Red Cell Membranes and Isolated Murine Mitochondria

摘要: Abstract The phototoxicity mechanism of a kryptocyanine dye, N,N′ -bis(2-ethyl-1,3-dioxolane)kryptocyanine (EDKC + ), has been studied in RBC membranes and isolated mitochondria. Lipophilic, positively charged dyes, such as EDKC , may be useful tumor-cell-selective, light-activated cytotoxic agents. Exposure the to 700-nm light inhibited membrane acetylcholinesterase photo-decomposed air-purged but not argon-purged samples. Photoinactivation was same D 2 O H quenched by superoxide dismutase. Ascorbate azide (10 mm) or slightly enhanced, respectively, inactivation. In samples containing methyl viologen, photodecomposed, activity unaffected. involve electron transfer oxygen subsequent formation toxic photoproducts from . contrast, exposure murine mitochondria caused inhibition mitochondrial respiration both presence absence oxygen. photodecomposition correlated with respiration. Thus, due photoexcited acceptors membrane. These studies indicate that dyes for photochemotherapy hypoxic regions tumors.