Behavior of tissue-engineered human cartilage after transplantation into nude mice.

摘要: Cartilage lacks the ability to regenerate structural defects. Therefore, autologous grafting has been used routinely replace cartilaginous lesions. Because tissue engineering of human cartilage with help bioresorbable polymer scaffolds is possible in experimental models, demand for clinical application grows. In this study we present an analysis behavior transplants made chondrocyte pools, agarose and resorbable scaffold Ethisorb a preliminary comparison single patients' cells but without additional ingredient agarose. Chondrocytes were isolated from matrix septal by enzymatic digestion. The pool kept monolayer culture 2 weeks, 3–4 weeks. Chondrocyte pools suspended seeded into Ethisorb. Single All cell–polymer constructs perfusion 10–14 days transplanted subcutaneously thymusaplastic nude mice. Additionally implanted fleeces embedded chondrocytes. After 6, 12 24 weeks animals sacrificed specimens explanted analyzed histochemically immunohistochemically. Polymer not chondrocytes did show formation. Resorption was complete after vivo. Transplants cell remained mechanically stable over apart four that resorbed completely. formation observed all pool-specimens presence chondronic structures homogeneous containing hyaline cartilage-specific molecules such as collagen type II. showed synthesis mechanical stability well. are suitable method vitro vivo models. Under conditions it is, however, necessary generation cells. We ingredients However, variations results more difficult than one would expect when using pools. Further studies need be performed find out which individual factors influence engineered cartilage's fate © 1999 Kluwer Academic Publishers