In situ gel-forming system for dual BMP-2 and 17β-estradiol controlled release for bone regeneration in osteoporotic rats.

摘要: The aim of this study was to evaluate the bone regeneration capacity a Pluronic® F127 (P)-Tetronic®1307 (T) and α-cyclodextrin (CD) supramolecular gel (P-T-CD) in 11/7/7 ratio containing BMP-2 17β-estradiol pre-encapsulated poly-lactide-co-glycolide (PLGA) poly-lactic acid (PLA-S) microspheres, respectively. Ovariectomy combined with dexamethasone treatment used induce an osteoporotic (OP) animal model calvaria critical size defect female rats test system. two active substances showed biphasic vitro release profile characterized by initial fast phase followed slow prolonged release. morphogenetic protein-2 (BMP-2) vivo faster than vitro. experimental design included four groups sham other OP treated blank system, BMP-2, one or doses 17β-estradiol. After 12 weeks, histological histomorphometric analyses that did not improve repair response sham, whereas animals, significant increase rate observed respect group alone. However, low values osteocalcin, immature poorly mineralized new animals. second dose system any case.