Rare-event analysis of circulating human dendritic cell subsets and their presumptive mouse counterparts.

摘要: Background. Considerable interest has focused recently on murine CD8α - and + dendritic cell (DC) subsets, because of their roles in initiating regulating immune responses. Attention also centered presumed human counterparts, DC1 DC2, respectively, precursors. Identification quantification these subsets the blood may be crucial to understanding monitoring immunologic significance, particularly humans, where only tissue readily or routinely available. Methods. Leukocytes were isolated from anticoagulated mouse (C57BL/10J) using conventional procedures. Four-color, rare-event, flow cytometric analysis was used identify precursors (pDC1; lineage [lin] CD4 CD11c HLA-DR ) DC2 (pDC2; lin CD123 hi [IL-3Rα ] normal humans. In mice, (CD11b lo , DC identified both animals after administration potent growth factor, fins-like tyrosine kinase 3 ligand (Flt3L). Results. All subjects examined had discrete populations pDC1 pDC2 comprising approximately 0.6% 0.1% mononuclear cells. constituted 0.75% 0.2%, cells 12% 0.5%, Flt3L-treated animals. Flt3L substantially increased absolute numbers circulating CD11C+ by 200-fold. Conclusions. addition pDCl DC, can blood, respectively. Monitoring isolation characterization provide novel insights into functional significance transplantation other clinical conditions.