Identification of Contact Allergens by In Vitro Cell Culture-Based Methods

摘要: If an in vitro model is to replace animal model, it should perform at least the same degree of accuracy as current models. Most importantly correlate human experience. Currently thought that no single assay will meet these requirements and a battery assays mimic sensitization be developed. It such cell-based situation more than assays. Assays reflecting epidermal penetration activation, dendritic cell maturation migration, T priming are being The irritant property sensitizer may related potency enable determined with aid equivalent assay. A proteomic approach investigate effect chemical sensitizers on keratinocyte metabolism identify novel biomarkers. Genomic studies identifying signatures which from non-sensitizers. In can used distinguish nature assays, however, makes currently unlikely they suitable for high-throughput screening. Progress toward validation slow but steadily moving forward. number identified pre-validation then studies.