A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids

摘要: Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while remains towards others. An OR deoxyribozyme logic gate was fluorescently report the of enterovirus 71 (EV71) covering ∼90% all known EV71 strains. Importantly, closely related coxsackieviruses differed by single nucleotides were reliably differentiated 7 out 8 cases.