Production of a composite hyaluronic acid/gelatin blood plasma gel for hydrogel-based adipose tissue engineering applications
作者： Esra Korurer , Halime Kenar , Emek Doger , Erdal Karaoz
DOI: 10.1002/JBM.A.34901
关键词:
摘要: Department of Gynecology and Obstetrics, Kocaeli University, Izmit Kocaeli, Turkey 41380Received 14 July 2013; accepted 22 2013Published online in Wiley Online Library (wileyonlinelibrary.com). DOI: 10.1002/jbm.a.34901Abstract: Standard approaches to soft-tissue reconstructioninclude autologous adipose tissue transplantation, but mostof the transferred is generally reabsorbed ashort time. To overcome this problem, long lasting implant-able hydrogel materials that can support regenerationmust be produced. The purpose study was evaluatethe suitability composite 3D natural origin scaffolds forreconstructive surgery applications through vitro tests. TheYoung’s modulus glutaraldehyde crosslinked hyal-uronic acid/gelatin (HA/G) plasma gels, composed humanplatelet-poor plasma, gelatin human umbilical cord acid, determined as 3.5 kPa, close softtissues. HA/G gels had higher porositythan plain (72.5% vs. 63.86%). Human adiposetissue derived stem cells (AD-MSCs) were isolated fromhuman lipoaspirates characterized with flow cytometry,and osteogenic adipogenic differentiation. Cell prolifera-tion assay AD-MSCs on revealed thenontoxic nature these constructs. Adipogenic differentia-tion distinctly better than plainplasma gels. results showed gelwith its suitable pore size, mechanical properties excel-lent cell growth adipogenesis supporting canserve a useful scaffold for engineeringapplications.
sci-hub.se 参考文章(38)
Steven M. Devine, Mesenchymal stem cells: Will they have a role in the clinic? Journal of Cellular Biochemistry. ,vol. 85, pp. 73- 79 ,(2002) , 10.1002/JCB.10046
Tony G van Tienen, Ralf G.J.C Heijkants, Pieter Buma, Jacqueline H de Groot, Albert J Pennings, Rene P.H Veth, Tissue ingrowth and degradation of two biodegradable porous polymers with different porosities and pore sizes. Biomaterials. ,vol. 23, pp. 1731- 1738 ,(2002) , 10.1016/S0142-9612(01)00280-0
Adam J. Katz, Ramon Llull, Marc H. Hedrick, J.W. Futrell, Emerging approaches to the tissue engineering of fat. Clinics in Plastic Surgery. ,vol. 26, pp. 587- 603 ,(1999) , 10.1016/S0094-1298(20)32659-6
Rowena McBeath, Dana M Pirone, Celeste M Nelson, Kiran Bhadriraju, Christopher S Chen, CELL SHAPE, CYTOSKELETAL TENSION, AND RHOA REGULATE STEM CELL LINEAGE COMMITMENT Developmental Cell. ,vol. 6, pp. 483- 495 ,(2004) , 10.1016/S1534-5807(04)00075-9
HG Shivakumar, CS Satish, KP Satish, Hydrogels as controlled drug delivery systems: Synthesis, crosslinking, water and drug transport mechanism Indian Journal of Pharmaceutical Sciences. ,vol. 68, pp. 133- 140 ,(2006) , 10.4103/0250-474X.25706
Anita Mol, Marjolein I. van Lieshout, Christa G. Dam-de Veen, Stefan Neuenschwander, Simon P. Hoerstrup, Frank P.T. Baaijens, Carlijn V.C. Bouten, Fibrin as a cell carrier in cardiovascular tissue engineering applications Biomaterials. ,vol. 26, pp. 3113- 3121 ,(2005) , 10.1016/J.BIOMATERIALS.2004.08.007
A. Vats, N.S. Tolley, J.M. Polak, J.E. Gough, Scaffolds and biomaterials for tissue engineering: a review of clinical applications Clinical Otolaryngology and Allied Sciences. ,vol. 28, pp. 165- 172 ,(2003) , 10.1046/J.1365-2273.2003.00686.X
Mark F Pittenger, Alastair M Mackay, Stephen C Beck, Rama K Jaiswal, Robin Douglas, Joseph D Mosca, Mark A Moorman, Donald W Simonetti, Stewart Craig, Daniel R Marshak, Multilineage Potential of Adult Human Mesenchymal Stem Cells Science. ,vol. 284, pp. 143- 147 ,(1999) , 10.1126/SCIENCE.284.5411.143
Culture of Cells for Tissue Engineering: Vunjak-Novakovic/Culture of Cells for Tissue Engineering John Wiley & Sons, Inc.. ,(2005) , 10.1002/0471741817
Gordana Vunjak-Novakovic, None, Culture of Cells for Tissue Engineering ,(2006)