Potential of mZD7349-conjugated PLGA nanoparticles for selective targeting of vascular cell-adhesion molecule-1 in inflamed endothelium
作者： Fatemeh Imanparast , Maliheh Paknejad , Mohammad Ali Faramarzi , Farzad Kobarfard , Amir Amani
DOI: 10.1016/J.MVR.2016.04.003
关键词:
摘要: Early diagnosis and restoring normal function of dysfunctional endothelium is an attractive strategy for prevention inflammatory diseases such as atherosclerosis. Inhibition cell adhesion in the process atherosclerosis plaque formation, mediated by peptide antagonists very late antigen-4 (VLA-4) has already been developed evaluated both vitro vivo. In this study, first time, modified ZD7349 (mZD7349) peptide, antagonist VLA-4, was used targeting fluorescein isothiocyanate-loaded poly (DL-lactic-co-glycolic acid) nanoparticles (FITC-PLGA NPs). Rate binding internalization mZD7349-NPs to activated human umbilical vein endothelial cells (HUVECs) were compared with that untargeted. Effects temperature reduction clathrin-mediated endocytosis inhibitor (0.45M sucrose) also studied on NPs. Results showed conjugated NPs could be significantly blocked pre-incubating free suggesting attaching surface VCAM-1 HUVECs. Also, FITC-loaded shown rapidly endocytosized a greater extent than unconjugated ones. The slowed down at low presence sucrose reductions mZD7349-NPs. To conclude, peptide-NPs suggested theranostic carrier lesions upregulating VCAM-1.
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