Vector systems for heterologous expression of proteins in Saccharomyces cerevisiae.

摘要: Publisher Summary The expression cassettes of the plasmids comprise a distinct promoter, cloning array with six to nine unique restriction sites, and CYC1 terminator. One class promoters used is considered be constitutive, including weakened ADH1 stronger TEF2 or GPD promoter. second includes regulatable MET25 which controlled by methionine repression, as well GALl subject strong glucose repression can induced galactose. Together, these vectors allow regulated coexpression up seven different proteins at various levels provide powerful tool for analysis dosage-dependent effects in S. cerevisiae. This chapter describes conversion several GATEWAY format based on this vector collection epitope-tagged proteins. properties some present destination vectors. discusses special emphasis effect epitope tags protein activity obtained heterologous green fluorescent (GFP).