Application of Glutamate in the Cortex of Rats: A Microdialysis Study
作者： B. Alessandri , H. Landolt , H. Langemann , J. Gregorin , J. Hall
DOI: 10.1007/978-3-7091-6894-3_2
关键词:
摘要: Glutamate, a major neurotransmitter in the brain, is also involved pathophysiological processes resulting secondary lesions following ischaemia or trauma. In present study we investigated relationship between glutamate excitotoxicity, free radical induction (indicated by ascorbic acid level) and glucose-lactate metabolism. Monosodium was applied through microdialysis probes (500 mM perfusate) into cortex of rats for 30 minutes (ASC), glucose (GLUC) lactate (LAC) were measured dialysates. Glutamate produced cortical lesion with an average volume 12.7 ± 1.4 mm3. Analysis dialysates revealed significant increase ASC (325 52% baseline) LAC (677 86%) core lesion. periphery nonsignificant short-lasting elevation both parameters second probe (about 1.3 mm frontally to first probe). A concomitant decrease GLUC found probes, reaching 29 8% 60 7% basal levels lesion, respectively. addition, studied delivery characteristics several concentrations (10, 100 1000 during 90-minute application cortex. The from perfusate brain about 33–38% min afterwards 11–25% total perfusate. results show that changes composition extracellular fluid, which could contribute development
springer.com
sci-hub.st 参考文章(31)
Benveniste H, The excitotoxin hypothesis in relation to cerebral ischemia. Cerebrovascular and brain metabolism reviews. ,vol. 3, pp. 213- 245 ,(1991)
L. Deecke, Eccles, John C. , Sir, H. H. Kornhuber, Vernon B. Mountcastle, From neuron to action : an appraisal of fundamental and clinical research Springer-Verlag. ,(1990)
DW Choi, M Maulucci-Gedde, AR Kriegstein, Glutamate neurotoxicity in cortical cell culture The Journal of Neuroscience. ,vol. 7, pp. 357- 368 ,(1987) , 10.1523/JNEUROSCI.07-02-00357.1987
L. K. Fellows, M. G. Boutelle, M. Fillenz, Physiological Stimulation Increases Nonoxidative Glucose Metabolism in the Brain of the Freely Moving Rat Journal of Neurochemistry. ,vol. 60, pp. 1258- 1263 ,(1993) , 10.1111/J.1471-4159.1993.TB03285.X
Richard M. Mangano, Robert Schwarcz, Chronic infusion of endogenous excitatory amino acids into rat striatum and hippocampus Brain Research Bulletin. ,vol. 10, pp. 47- 51 ,(1983) , 10.1016/0361-9230(83)90073-4
Raymond A. Swanson, Jun Chen, Steven H. Graham, Glucose Can Fuel Glutamate Uptake in Ischemic Brain Journal of Cerebral Blood Flow and Metabolism. ,vol. 14, pp. 1- 6 ,(1994) , 10.1038/JCBFM.1994.1
David Nicholls, David Attwell, The release and uptake of excitatory amino acids. Trends in Pharmacological Sciences. ,vol. 11, pp. 462- 468 ,(1990) , 10.1016/0165-6147(90)90129-V
L. K. Fellows, M. G. Boutelle, M. Fillenz, Extracellular brain glucose levels reflect local neuronal activity: a microdialysis study in awake, freely moving rats. Journal of Neurochemistry. ,vol. 59, pp. 2141- 2147 ,(2006) , 10.1111/J.1471-4159.1992.TB10105.X
F. Staub, A. Baethmann, J. Peters, H. Weigt, O. Kempski, Effects of Lactacidosis on Glial Cell Volume and Viability Journal of Cerebral Blood Flow and Metabolism. ,vol. 10, pp. 866- 876 ,(1990) , 10.1038/JCBFM.1990.143
Philippe Lyrer, Hans Landolt, Alexa Kabiersch, Helen Langemann, Heinrich Kaeser, Levels of low molecular weight scavengers in the rat brain during focal ischemia Brain Research. ,vol. 567, pp. 317- 320 ,(1991) , 10.1016/0006-8993(91)90811-9