High-Throughput Screening of Protein Interaction Networks in the TGFβ Interactome: Understanding the Signaling Mechanisms Driving Tumor Progression

摘要: High-throughput (HT) proteomic techniques allow the study of hundreds to thousands proteins simultaneously. Several HT methodologies have been developed determine protein-protein interactions (PPIs) and therefore protein function in mammalian cells. A few these, including complementation assays, mass spectrometry, yeast two-hybrid luminescence-based interactome (LUMIER) mapping, applied TGFβ signaling. PPIs revealed with these crucial elucidating novel components signaling network involved tissue homeostasis cancer. good example this is recently described TGFβ/Par6 polarity pathway, which was initially discovered a LUMIER screen for PPIs. role pathway process epithelial-mesenchymal transition has demonstrated, suggesting its potential involvement cancer metastasis. Thus, data are becoming an essential tool unraveling dynamic networks that drive onset tumor progression.