Molecular Techniques for DNA Methylation Studies

摘要: Abstract DNA methylation is the best-studied epigenetic modification and altered patterns have been identified in cancer many other complex diseases. The first methylation-based biomarkers for early diagnosis of approved by regulatory agencies, which illustrate potential advantages analysis. A number technologies devised to study genome-wide or at specific loci using a limited principles differentiating state (methylation-sensitive/dependent restriction enzymes, antibody methyl-binding protein, bisulfite conversion). choice best-suited assay depends on biological question be investigated, quantity quality material available, access instrumentation. For discovery patterns, second-generation sequencing has largely replaced microarrays as read-out platform whereas locus-specific analysis, robust quantitative are available that deliver accurate profiles single-nucleotide resolution. In this chapter, currently used methods well analysis 5-methylcytosine reviewed detail limitations each approach discussed.