NMR Spectroscopic Characterization of Sarcolemmal Permeability During Myocardial Ischemia and Reperfusion

摘要: This study aims to characterize the pattern of membrane disintegration during myocardial ischemia and reperfusion. Intracellular volumes were measured by 1H 59Co NMR in isolated rat hearts 10, 30 60 min total reperfusion at normothermia. Perfusion with hypo-osmotic medium (210 mosm/l) increased intracellular water from 2.50+/-0.06 3.07+/-0.07 ml/g dry weight (P<0.001) pre-ischemia. Hypo-osmotic swelling decreased 16+/-3, 32+/-6 44+/-11% pre-ischemic value after (n.s., P<0.005, P<0.001) respectively, indicating that permeabilization facilitated efflux osmolytes counterbalanced osmotic driving force for influx. 18+/-5% all groups (P<0.0.005 v post-ischemia). The volume distribution extracellular marker cobalticyanide more than 3.2+/-0.4 5.8+/-0.5% space respectively (P<0.001), an additional 2% During reperfusion, released 1.6+/-0.2 3.2+/-0.4% creatine kinase contents ischemia, (P<0.001). In addition correlation between duration permeability, evident analysis each probe, data showed a progressive increase severity injury over time larger molecules. 23Na spectroscopy conjunction shift reagent (SR) formation resonance intermediate chemical intra Na+ peaks, suggesting penetration SR into cells disrupted membranes. constant narrow line shape this resonance, characteristic homogeneous environment, suggested was contained within cytosol cardiomyocytes. We propose sarcolemmal membranes are gradually permeabilized molecules evolving instability is spatially myocyte.