The gap junction channel. Its aqueous nature as indicated by deuterium oxide effects.

摘要: The effects of temperature and solvent substitution with deuterium oxide (D2O) on axoplasmic (ga) gap junctional (gj) conductances were examined in the earthworm septate median giant axon (MGA). coefficients (Q10) for ga gj 1.4 1.5, respectively, between 5 15 degrees C. Substitution D2O rapidly reduced both by 20% increased Q10's to 1.5 1.8, respectively. reduction upon cooling either reflects changes that occur viscosity, which indicates ion mobility axoplasm, as free solution, is primarily governed viscous properties solvent. similar initial observed suggests occupies junction channel volume influences transjunctional mobility. With time there was a further at 20 C larger Q10 D2O. enhanced cannot be accounted viscosity alone may due an hydration channels and/or transport ions isotope hydrogen-deuterium exchange protein reduce gj.