Maximization of hydrogen production ability in high‐density suspension of Rhodovulum sulfidophilum cells using intracellular poly(3‐hydroxybutyrate) as sole substrate

摘要: Growth of and hydrogen production by wild-type (WT) Rhodovulum sulfidophilum were compared with those one its mutants lacking the poly(3-hydroxybutyrate) (PHB) biosynthesis ability (PNM2). During phototrophic growth under aerobic conditions fixed illumination, changes in extinction coefficient PHB content WT PNM2 cells revealed interference light penetration PHB. synthesized at an early stage cultivation. degradation after exhaustion acetate during cultivation resulted a decrease coefficient. The rate anaerobic illumination was examined cell suspensions different densities. determined not but kinds donors density suspension. highest value from PHB, 33.0 ml/l/h, improved 26.6 which succinate. Under same conversion to is more efficient than that succinate, best substrates for production. These results suggest can be maximized system based on degradation, achieved high-density suspension external-substrate-depleted presence excess amount acetate.