Recombinant bacteria expressing functional r76 mammalian receptors on their surface
作者： Laurent Emorine , Colette Delavier , Stefano Marullo , Donny Strosberg
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摘要: The invention relates to a vector capable of being replicated in cultures unicellular organisms, this containing gene coding for eucaryotic protein having the biological activity membrane receptor and interacting with regulatory protein--called G protein--able bind molecules guanosine triphosphate (GTP). also cell organisms transformed by above vectors. It procedures detection capacity molecule behave as ligand procedure studying affinity well kit detecting possible receptor.
lens.org 参考文章(16)
S. Marullo, L.J. Emorine, A.D. Strosberg, C. Delavier-Klutchko, Selective binding of ligands to beta 1, beta 2 or chimeric beta 1/beta 2-adrenergic receptors involves multiple subsites. The EMBO Journal. ,vol. 9, pp. 1471- 1476 ,(1990) , 10.1002/J.1460-2075.1990.TB08264.X
B K Kobilka, T Frielle, H G Dohlman, M A Bolanowski, R A Dixon, P Keller, M G Caron, R J Lefkowitz, Delineation of the intronless nature of the genes for the human and hamster beta 2-adrenergic receptor and their putative promoter regions. Journal of Biological Chemistry. ,vol. 262, pp. 7321- 7327 ,(1987) , 10.1016/S0021-9258(18)48239-7
Y Yarden, Hu Rodriguez, SK Wong, DR Brandt, DC May, J Burnier, RN Harkins, EY Chen, J Ramachandran, A Ullrich, None, The avian beta-adrenergic receptor: primary structure and membrane topology. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 6795- 6799 ,(1986) , 10.1073/PNAS.83.18.6795
Gabriella Galli, Raffaele Matteoni, Emma Bianchi, Luana Testa, Daniela Marazziti, Nicoletta Rossi, Glauco Tocchini-Valentini, Replica filter assay of human β-adrenergic receptors expressed in E. coli Biochemical and Biophysical Research Communications. ,vol. 173, pp. 680- 688 ,(1990) , 10.1016/S0006-291X(05)80089-0
T. Frielle, S. Collins, K. W. Daniel, M. G. Caron, R. J. Lefkowitz, B. K. Kobilka, Cloning of the cDNA for the human beta 1-adrenergic receptor. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 7920- 7924 ,(1987) , 10.1073/PNAS.84.22.7920
L. J. Emorine, S. Marullo, C. Delavier-Klutchko, S. V. Kaveri, O. Durieu-Trautmann, A. D. Strosberg, Structure of the gene for human beta 2-adrenergic receptor: expression and promoter characterization. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 6995- 6999 ,(1987) , 10.1073/PNAS.84.20.6995
Henrik G. Dohlman, Marc G. Caron, Robert J. Lefkowitz, A family of receptors coupled to guanine nucleotide regulatory proteins. Biochemistry. ,vol. 26, pp. 2657- 2664 ,(1987) , 10.1021/BI00384A001
W. H. M. L. Luyten, P. J. Pauwels, H. Moereels, S. Marullo, A. D. Strosberg, J. E. Leysen, Comparative Study of the Binding Properties of Cloned Human β1- and β2-Adrenergic Receptors Expressed in Escherichia coli Drug Investigation. ,vol. 3, pp. 3- 12 ,(1991) , 10.1007/BF03258257
B. Kobilka, H Matsui, T. Kobilka, T. Yang-Feng, U Francke, M. Caron, R. Lefkowitz, J. Regan, Cloning, sequencing, and expression of the gene coding for the human platelet alpha 2-adrenergic receptor Science. ,vol. 238, pp. 650- 656 ,(1987) , 10.1126/SCIENCE.2823383
Edwin S. Levitan, Cloning of serotonin and substance K receptors by functional expression in frog oocytes. Trends in Neurosciences. ,vol. 11, pp. 41- 43 ,(1988) , 10.1016/0166-2236(88)90159-2