Molecular identification of Trichoderma species associated with Pleurotus ostreatus and natural substrates of the oyster mushroom.

摘要: Green mold of Pleurotus ostreatus, caused by Trichoderma species, has recently resulted in crop losses worldwide. Therefore, there is an emerging need for rapid means diagnosing the causal agents. A PCR assay was developed detection pleurotum and pleuroticola, two pathogens causing green P. ostreatus. Three oligonucleotide primers were designed identifying these species a multiplex based on DNA sequences within fourth fifth introns translation elongation factor 1α gene. The detected presence T. and/or pleuroticola directly growing substrates oyster mushrooms, without isolating pathogens. used to assess natural environments which ostreatus can be found Hungary, demonstrated that present surface basidiomes wild mushrooms. Other habitats harzianum, longibrachiatum atroviride. not any samples from forested areas tested this study.