Influence of Polyethylene Glycol on Leaf Anatomy, Stomatal Behavior, Water Loss, and Some Physiological Traits of Date Palm Plantlets Grown In Vitro and Ex Vitro.

摘要: Few reports explain the mechanism of PEG action on stomatal behavior and anatomical structure analyze photosynthetic pigments in vitro date palm plantlets for better tolerance to ex exposure. The main challenge micropropagation techniques remains restricted high survival rates vigorous growth after transplantation. In hardening is induced by Polyethylene glycol (0.0, 10, 20, 30 g L−1) 4 weeks. Leaf anatomy, behavior, water loss %, pigments, reducing sugars were examined (Phoenix dactylifera L.) cv. (Sewi) weeks from treatment transplanting greenhouse. anatomy surface ultrastructure untreated leaves showed a thin cuticle layer, wide opened malfunctioning stomata, abnormal leaf anatomy. Furthermore, addition resulted increasing thickness, epicuticular wax depositions, plastids density, improving ability close decreasing aperture length while substomatal chambers intercellular spaces mesophyll. As result, significant reduction % was observed both treated as compared ones, which exhibited rapid wilting when exposed low humidity h. application significantly increased Chlorophylls a, b carotenoids concentrations, especially 20 L−1 treatments, sequentially reflected sugar concentration. However, with at became yellow had necrosis ends death. rate 90% transfer 63% recorded plantlets. Therefore, this provides normal developed faster enhances transfer.