Antibiotic induced biofilm formation of novel multidrug resistant Acinetobacter baumannii ST2121 clone.
作者： Müberra Çimen , Azer Özad Düzgün
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摘要: The aim of this study was to identify antimicrobial resistance and virulence factor genes exhibited by multidrug resistant (MDR) Acinetobacter baumannii, analyze biofilm formation investigate clonal subtypes isolate. Whole genome sequencing done Illumina NovaSeq 6,000 platform multilocus sequence typing (MLST) performed Oxford Pasteur schemes. Influence imipenem levofloxacin on investigated in 96-well plates at 3 replicates. strain found carry OXA-23, OXA-51-like, AmpC TEM-1 beta-lactamases. the blaOXA-51-like gene has been identified as a blaOXA-66. According MLST scheme displayed ST2 allelic profile. However, based represents new ST2121, gdhB single mutation namely, gdhB-227. It determined that MDR isolate carried bap, basABCDFGHIJ, csuA/BABCDE, bauABCDEF, plcD, pgaABCD, entE, barAB, ompA, abaIR, piT2EAFTE/AUBl, fimADT, cvaC, bfmR, bfmS genes. In our induced highest concentration 32 µg/ml 16 µg/ml. conclusion, we detected A. baumannii ST2121 clone harboring blaOXA-66 reported for first time Turkey.
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