Association of filamin A and vimentin with hepatitis C virus proteins in infected human hepatocytes.

摘要: Chronic hepatitis C (CHC) infection caused by virus (HCV) is a major cause of liver disease and remains therapeutic challenge. A variety host proteins interact with HCV proteins. The definitive role cytoskeletal (CS) in to be determined. In this study, our aim was determine the expression profile differentially regulated expressed selected CS their association infected hepatocytes as possible targets. Using proteomics, qRT-PCR, Western blot immunofluorescence techniques, we revealed that filamin (fila) vimentin (vim) were prominently increased HCV-expressing human hepatoma cells compared parental biopsies from patients CHC vs controls. nonstructural (NS) 3 NS5A associated fila, while core protein partially fila vim. Immunoprecipitation showed interactions among NS3 Cells treated interferon-α dose- time-dependent decrease NS clustered at perinuclear region following cytochalasin b treatment, whereas disperse cytoplasmic distribution observed no-treatment group. This study demonstrates signifies changes occur HCV-infected and, for first time, shows up-regulation interaction Association between may have implications future design protein-targeted therapy treatment infection.