Identification and drug sensitivity of a Plesiomonas shigelloides isolated from diseased sturgeons

摘要: Objective In 2012, the cumulative mortality of farmed sturgeons in Beijing was almost 60% with various symptoms, including reddening anus yellow exudation, ascities peritoneal cavity, petechial haemorrhages liver and internal muscle wall, swollen spleen. Methods We isolated pathogen from dying significant pathological signs, then analyzed its morphological, physiological biochemical characteristics, taxonomic status, drug sensitivity. Moreover, pathogenic characteristic presumptive pathogens identified by artificial infection. Results The 16S rDNA sequence more than 99% homology that Plesiomonas shigelloides, suggesting P. which also demonstrated results tests. LD50 to sturgeon 1.0 x 10(5.8) CFU/mL, it can cause liver, kidey spleen lesions. There were no activities amylase, caseinase, lipase, gelatinase haemolysis extracellular products toxicity might be endotoxin. addition, bacterium specific sensitive enrofloxacin, doxycyline hyclate, florfenicol thiamphenicol MIC less 2 microg/mL. Conclusion shigelloides main cultured area, hyclate used against disease.