Co-expression of vesicular glutamate transporters (VGLUT1 and VGLUT2) and their association with synaptic-like microvesicles in rat pinealocytes

摘要: A vesicular glutamate transporter (VGLUT) is responsible for the accumulation of l-glutamate in synaptic vesicles glutamatergic neurons. Two isoforms, VGLUT1 and VGLUT2, have been identified, which are complementarily expressed these Mammalian pinealocytes, endocrine cells melatonin, also nature, accumulate synaptic-like microvesicles (SLMVs), secrete it through exocytosis. Although storage SLMVs mediated a VGLUT, molecular nature less understood. We recently observed that VGLUT2 pinealocytes. In present study, we show pinealocytes express VGLUT1. RT-PCR northern blot analyses indicated expression gene pineal gland. Western blotting with specific antibodies against presence Indirect immunofluorescence microscopy section gland cultured co-localized process terminal regions Furthermore, immunoelectronmicroscopy as well subcellular fractionation studies revealed both specifically associated SLMVs. These results indicate VGLUTs Pinealocytes first exception to complementary VGLUT2.