Synergism and Substitution in the Lactoferrins
作者： Andrew M. Brodie , Eric W. Ainscough , Edward N. Baker , Heather M. Baker , Musa S. Shongwe
DOI: 10.1007/978-1-4615-2548-6_4
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摘要: The anion binding properties of human lactoferrin (Lf), with Fe3+ or Cu2+ as the associated metal ion, highlight differences between two sites, and in behaviour when different metals are bound. Carbonate, oxalate hybrid carbonate-oxalate complexes have been prepared their characteristic electronic EPR spectra recorded. Oxalate can displace carbonate from either one both sites Cu2(CO3)2Lf, depending on concentration, but no such displacement occurs for Fe2(CO3)2Lf although it does bovine analogue. Addition appropriate ion to apoLf under carbonate-free conditions gives dioxalate Cu2+. determined crystal structures Fe2(CO3)2Lf, Fe2(C2O4)2Lf, Cu2(CO3)(C2O4)Lf compared. Both ions bind bidentate fashion metal, except that N-lobe site dicupric is monodentate. copper complex shows binds preferentially C-lobe a mode. A series containing synergistic O,N-chelates increasing substitution N atom (glycinate, iminodiacetate nitrilotriacetate) iron comparison O,O-chelate oxalate. Overall these observations lead generalised model by transferons allow comparisons be made nonsynergistic anions citrate succinate.
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