Spontaneous Transformation of Rat Ovarian Surface Epithelial Cells Results in Well to Poorly Differentiated Tumors with a Parallel Range of Cytogenetic Complexity

摘要: We previously used rat ovarian surface epithelial cells subjected to repetitious growth in vitro provide experimental evidence support of a role for incessant ovulation the etiology cancer. have now initiated series 30 independent cell lines. This report describes findings eight lines that, based on tumor formation athymic mice, undergone malignant transformation. Each tumors exhibited chromosomal alterations. Two well moderately differentiated had only one or two cytogenetic changes, and they common presence numerical gains. five poorly complex karyotypes with three clonal aberrations, prominent among them being unbalanced rearrangements losses. Several also marker chromosomes, double minutes, homogeneously staining regions. These demonstrate that produced by spontaneously transformed range degree differentiation, which is paralleled complexity. Thus, this model system may fill an important void future efforts define genetic basis ovary many features characteristic these neoplasms.