T-DNA from Agrobacterium tumefaciens as an efficient tool for gene targeting in Kluyveromyces lactis.
作者： P. Bundock , K. Mróczek , A. A. Winkler , H. Y. Steensma , P. J. J. Hooykaas
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摘要: … contained this T-DNA construct integrated by homologous recombination at the KlTRP1 locus. Gene replacement events were the most common. In contrast, 82% of the transformants …
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