Light-Dependent Isoprene Emission (Characterization of a Thylakoid-Bound Isoprene Synthase in Salix discolor Chloroplasts)

摘要: Isoprene synthase is an enzyme that responsible for the production of volatile C5 hydrocarbon, isoprene, in plant leaves. formation numerous C3 plants interesting because (a) large quantities isoprene are emitted, 5 x 1014 g C annually, (b) a may release 1 to 8% its fixed as and (c) function unknown. Because dependence foliar emission on light, existence plastidic has been postulated. To pursue this idea, method isolate chloroplasts from Salix discolor was developed shows tightly bound thylakoid membrane accessible trypsin inactivation. The thylakoid-bound catalytic properties similar known soluble synthases; however, relationship between these enzymes discovery with stromal-facing domain places it chloroplast, where be subject direct indirect light-mediated effects. Implications light-dependent regulation presented.