THE SACCHAROMYCES CEREVISIAE MEVALONATE DIPHOSPHATE DECARBOXYLASE IS ESSENTIAL FOR VIABILITY, AND A SINGLE LEU-TO-PRO MUTATION IN A CONSERVED SEQUENCE LEADS TO THERMOSENSITIVITY

摘要: The mevalonate diphosphate decarboxylase is an enzyme which converts to isopentenyl diphosphate, the building block of isoprenoids. We used Saccharomyces cerevisiae temperature-sensitive mutant defective for previously described (C. Chambon, V. Ladeveve, M. Servouse, L. Blanchard, C. Javelot, B. Vladescu, and F. Karst, Lipids 26:633-636, 1991) characterize mutated allele. showed that a single change in conserved amino acid accounts phenotype mutant. Complementation experiments were done both erg19-mutated background strain ERG19 gene, was shown be essential gene yeast, disrupted. Epitope tagging wild-type allowed us isolate active form by versatile one-step immunoprecipitation procedure. Furthermore, during course this study, we observed high level expression led lower sterol steady-state accumulation compared strain, suggesting may key pathway regulation.