Determination of heparin and heparin-like substances in thalassemia patients with and without epistaxis using a novel monoclonal antibody
作者： Prachya Kongtawelert , Panja Kulapongs
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摘要: A monoclonal antibody to polysulphated polysaccharides was prepared and used in a plasma assay for heparin. The reacted with heparin semisynthetic heparin-like substances including dextran sulphate, pentosan polysulphate, glycosaminoglycan polysulphate.The analytical method biotin-labeled competitive enzyme linked immunosorbent (ELISA). level of obtained by the correlated those based on inhibition Factor Xa, but were numerically different (y = -0.1747 + 1.0848x; π2 0.844). could detect solution at submicrogram or milliunit level, which suggested that it suitable pharmacokinetic studies hepain polysulfaled polysaccharides. concentrations from thalassemic patients, either without epitaxis, not statistically normal subjects. Chiang Mai Med Bull 2000; (3-4): 57-66.
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