Catalyst mediated competitive protein binding assay

摘要: Method and compositions are provided for competitive protein binding assays wherein a non-enzymic catalytically mediated reaction is sterically inhibited by receptors to ligand which the intermediary bound. The method employs first second reactants, only react with each other either very slowly or not at all. catalyst provides two step having as its final result transformation of reactants. By conjugating ligand, whereby presence receptor bound inhibits approach one both antiligand can be determined. Alternatively, anticatalyst employed from when ligand. reaction. comparing rate an unknown amount analyte present rates known amounts analyte, concentration in may