Identification of an Attractin-Like Pheromone in the Mucus-Secreting Hypobranchial Gland of the AbaloneHaliotis asininaLinnaeus
作者： Chitraporn Kuanpradit , Scott F. Cummins , Bernard M. Degnan , Prapee Sretarugsa , Peter J. Hanna
DOI: 10.2983/035.029.0321
关键词:
摘要: Pheromones are chemicals used to communicate between animals of the same species, and thought be by most marine animals. With limited vision, abalone primarily sense their world chemically, pheromones may play an important role in settlement, attraction, recognition, alarm, reproduction. Despite this, there has been no detailed investigation into pheromone substances, both precise biochemical nature or pheromonal function. In this study, we investigated presence pheromonelike substances from hypobranchial gland Haliotis asinina using bioassays, immunohistochemistry, Western blotting, reverse-phase high-performance liquid chromatography (RP-HPLC). The many prosobranchial molluscs classified as a sex auxiliary releasing unknown during spawning. our cephalic tentacle assays demonstrated that cell extracts contain chemical cues sensed conspecifics. An antibody against sea slug “attractin” was probe localize similar protein mucin-secreting cells epithelial lining male female abalone. approximate molecular weight attractin-like is 30 kDa males females. Fractionation C5 RP-HPLC could not selectively purify protein, sex-specific differences were observed. We predict one number proteins involved maturation, aggregation, and/or spawning behavior future research, additional components will tested further for these types behavior.
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