Regulation by the antioxidants ascorbate, cysteine, and dapsone of the increased extracellular and intracellular generation of reactive oxidants by activated phagocytes from cigarette smokers.

作者: Annette J. Theron , Ronald Anderson , Gerhard J. Ras

DOI: 10.1164/ARRD.1987.135.5.1027

关键词:

摘要: The bimodal pattern of N-formyl-methionyl-leucyl-phenylalanine (FMLP)-activated luminol-enhanced chemiluminescence with distinct early (occurring within 1 min) extracellular and late intracellular oxidative responses was compared in polymorphonuclear leukocytes (PMNL) from asymptomatic cigarette smokers nonsmoking control subjects. Relative to PMNL, the PMNL were hyperreactive FMLP stimulation increased generation both (p less than 0.025) reactive oxidants. Smokers' also showed PMNL-activated superoxide apparent receptors for FMLP. water-soluble antioxidants ascorbate cysteine (2.5 X 10(-5) M 2.5 10(-4) M) selectively neutralized activity PMNL-derived lipid-soluble antioxidant dapsone (1.25 30 micrograms/ml), on other hand, inhibited FMLP-activated Regulation membrane-associated is probably an important function vivo.

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