Preparative isolation and purification of steroidal glycoalkaloid from the ripe berries of Solanum nigrum L. by preparative HPLC-MS and UHPLC-TOF-MS/MS and its anti-non-small cell lung tumors effects in vitro and in vivo.

摘要: Overcoming epidermal growth factor receptor resistance is a critical problem that needs to be solved in clinical practice. Drugs downregulate the fatty acid synthase-epidermal will become novel treatments for non-small cell lung cancer. Solanum nigrum, extracted with water at 4°C, shows strong cytotoxic activity and inhibits tumor Lewis bearing-mice dose-dependent manner. A active compound S. solaoiacid, was successfully separated purified from nigrum by preparative high-performance liquid chromatography mass spectrometry ultra high performance time-of-flight tandem spectrometry. The IC50 of solaoiacid on cancer cells 2.3 µmol/L, which significantly lower than known steroidal glycoalkaloid. Label-free proteomics STRING Network analysis were used identify deregulated proteins treated fresh ripe fruit extracts nigrum. regulates multiple signal pathways, including pathway. downregulated 24 main direct roles biosynthesis. Both showed downregulation anti-non-small effects, thus drug treatment