The effects of non-medically used psychoactive drugs on monoamine neurotransmission in rat brain.

摘要: We developed a reproducible, simple, and small-scale method for determining the re-uptake release of monoamines (dopamine, serotonin (5-HT) norepinephrine) using rat brain synaptosomes. These assays were then applied to study effects different kinds non-medically used psychoactive drugs on monoamine release. The phenethylamine derivatives, 4-fluoroamphetamine, 2-methylamino-3,4-methylene-dioxy-propiophenone (methylone), 1-(1,3-benzodioxol-5-yl)-2-butanamine (BDB), N-methyl-1-(1,3-benzodioxol-5-yl)-2-butanamine (MBDB), had strong inhibitory dopamine, 5-HT norepinephrine. 4-Fluoroamphetamine, methylone BDB also strongly increased three monoamines, but MBDB norepinephrine release, little effect dopamine However, 2,5-dimethoxy-4-iodophenethylamine (2C-I), 2,5-dimethoxy-4-ethylphenethylamine (2C-E), 2,5-dimethoxy-4-chlorophenethylamine (2C-C), 2,4,5-trimethoxyamphetamine (TMA-2) 2,4,6-trimethoxyamphetamine (TMA-6), which are methoxylated slightly influenced monoamines. Alpha-metyltryptamine (AMT), tryptamine derivative, was one strongest inhibitors releasers 5-methoxy-alpha-methyltryptamine (5-MeO-AMT), inhibited N,N-dipropyltryptamine (DPT), 5-methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT), 5-methoxy-N,N-methylisopropyltryptamine (5-MeO-MIPT), 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) re-uptake, few 1-(3-Chlorophenyl)piperazine (3CPP) 1-(methoxyphenyl)piperazine (4MPP), piperazine accelerated their results suggest that some designer act central nerve system same extent as restricted drugs.