A rapid, quantitative assay for direct detection of microRNAs and other small RNAs using splinted ligation.
作者： P. A. Maroney , S. Chamnongpol , F. Souret , T. W. Nilsen
DOI: 10.1261/RNA.518107
关键词:
摘要: The discovery and characterization of microRNAs (miRNAs) other families short RNAs has led to a rapid expansion research directed at elucidating their expression patterns regulatory functions. Here, we describe convenient, sensitive, straightforward method detect quantitate specific miRNA levels in unfractionated total RNA samples. method, based on splinted ligation, does not require specialized equipment or any amplification step, is significantly faster more sensitive than Northern blotting. We demonstrate that the can be used various classes small from different organisms.
springer.com
rnajournal.org 参考文章(14)
Melissa J. Moore, Charles C. Query, Joining of RNAs by splinted ligation. Methods in Enzymology. ,vol. 317, pp. 109- 123 ,(2000) , 10.1016/S0076-6879(00)17009-0
David P Bartel, MicroRNAs: Genomics, Biogenesis, Mechanism, and Function Cell. ,vol. 116, pp. 281- 297 ,(2004) , 10.1016/S0092-8674(04)00045-5
Alexander Hüttenhofer, Jörg Vogel, Experimental approaches to identify non-coding RNAs Nucleic Acids Research. ,vol. 34, pp. 635- 646 ,(2006) , 10.1093/NAR/GKJ469
Anna Válóczi, Csaba Hornyik, Nora Varga, József Burgyán, Sakari Kauppinen, Zoltan Havelda, Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes Nucleic Acids Research. ,vol. 32, ,(2004) , 10.1093/NAR/GNH171
Patricia A Maroney, Sangpen Chamnongpol, Frédéric Souret, Timothy W Nilsen, Direct detection of small RNAs using splinted ligation Nature Protocols. ,vol. 3, pp. 279- 287 ,(2008) , 10.1038/NPROT.2007.530
R. S. PILLAI, MicroRNA function: multiple mechanisms for a tiny RNA? RNA. ,vol. 11, pp. 1753- 1761 ,(2005) , 10.1261/RNA.2248605
Patricia A Maroney, Yang Yu, Jesse Fisher, Timothy W Nilsen, Evidence that microRNAs are associated with translating messenger RNAs in human cells Nature Structural & Molecular Biology. ,vol. 13, pp. 1102- 1107 ,(2006) , 10.1038/NSMB1174
Søren Peter Jonstrup, Jørn Koch, Jørgen Kjems, A microRNA detection system based on padlock probes and rolling circle amplification RNA. ,vol. 12, pp. 1747- 1752 ,(2006) , 10.1261/RNA.110706
Jinmai Jiang, Eun Joo Lee, Yuriy Gusev, Thomas D Schmittgen, Real-time expression profiling of microRNA precursors in human cancer cell lines Nucleic Acids Research. ,vol. 33, pp. 5394- 5403 ,(2005) , 10.1093/NAR/GKI863
Mariana Lagos-Quintana, Reinhard Rauhut, Winfried Lendeckel, Thomas Tuschl, Identification of Novel Genes Coding for Small Expressed RNAs Science. ,vol. 294, pp. 853- 858 ,(2001) , 10.1126/SCIENCE.1064921